Review





Similar Products

primary antibodies against p perk  (Bioss)
95
Bioss primary antibodies against p perk
Primary Antibodies Against P Perk, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibody+against+perk/PERK(Thr980)+Polyclonal+Antibody/pmc13096461-64-0-10
Average 95 stars, based on 1 article reviews
primary antibodies against p perk - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier
antibodies against p perk  (Bioss)
95
Bioss antibodies against p perk
Antibodies Against P Perk, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibody+against+perk/PERK(Thr980)+Polyclonal+Antibody/pm39326362-74-0-14
Average 95 stars, based on 1 article reviews
antibodies against p perk - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier
rabbit polyclonal antibody against p perk  (Proteintech)
96
Proteintech rabbit polyclonal antibody against p perk
Rabbit Polyclonal Antibody Against P Perk, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibody+against+perk/PERK%2FEIF2AK3+Antibody/pm38513524-45-14-19
Average 96 stars, based on 1 article reviews
rabbit polyclonal antibody against p perk - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
rabbit polyclonal antibody against perk  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc rabbit polyclonal antibody against perk
Rabbit Polyclonal Antibody Against Perk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibody+against+perk/primary+antibodies/pmc10706857-139-21-27
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibody against perk - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rabbit polyclonal antibodies against pp38, pjnk, and perk d3f9, 81e11 and d13.14.4e  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc rabbit polyclonal antibodies against pp38, pjnk, and perk d3f9, 81e11 and d13.14.4e
LPS activated MTX-treated M-MØ exhibit a functional proinflammatory profile. a Experimental design. Monocytes were untreated or exposed to 5 µM MTX at the beginning of the 7-day macrophage differentiation process with M-CSF and challenged with LPS (10 ng/mL) on day 7. Cells were assayed 3 h post-LPS stimulation on M-MØ and MTX-M-MØ. b Production of IL-10, IFNβ, and IL-6 by M-MØ and MTX-M-MØ challenged with LPS for 3 h as determined by ELISA. Mean ± SEM of 10–12 independent donors, each symbol represents a single donor (** p < 0.01, *** p < 0.001, paired t test). c Upper panel, scatter plot of RNAseq results showing gene expression changes 3 h post-LPS stimulation in MTX-M-MØ (LPS + MTX-M-MØ/LPS + M-MØ). The number of annotated genes whose expression is upregulated or downregulated 3 h post-LPS stimulation in M-MØ after 7 days of MTX treatment (adjusted p <0.05) is shown. Lower panel, relative level of expression of the indicated genes as determined by RNAseq on LPS + M-MØ and LPS + MTX-M-MØ, adjusted p value is indicated. d GSEA on the ranked comparison of the transcriptome of LPS + MTX-M-MØ versus LPS + M-MØ, using the genes significantly modulated by LPS in GM-MØ (GM-MØ-specific LPS-induced) and M-MØ (M-MØ-specific LPS-induced) as data set. The genes within the leading edge of each GSEA are indicated in online supplementary Table . e, f Immunoblot analysis of pERK, <t>pJNK,</t> <t>and</t> <t>pp38</t> ( e ), pIRF3 and pSTAT3 ( f ) by monocytes differentiated with M-CSF in the absence or presence of MTX for 7 days and challenged with LPS for the indicated time points. Vinculin or GAPDH protein levels were determined as protein loading control.
Rabbit Polyclonal Antibodies Against Pp38, Pjnk, And Perk D3f9, 81e11 And D13.14.4e, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibody+against+perk/sb203580/pmc10643894-53-11-19
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibodies against pp38, pjnk, and perk d3f9, 81e11 and d13.14.4e - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
polyclonal primary antibodies against pstat3 (tyr705), p-p38, perks, pjnks  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc polyclonal primary antibodies against pstat3 (tyr705), p-p38, perks, pjnks
LPS activated MTX-treated M-MØ exhibit a functional proinflammatory profile. a Experimental design. Monocytes were untreated or exposed to 5 µM MTX at the beginning of the 7-day macrophage differentiation process with M-CSF and challenged with LPS (10 ng/mL) on day 7. Cells were assayed 3 h post-LPS stimulation on M-MØ and MTX-M-MØ. b Production of IL-10, IFNβ, and IL-6 by M-MØ and MTX-M-MØ challenged with LPS for 3 h as determined by ELISA. Mean ± SEM of 10–12 independent donors, each symbol represents a single donor (** p < 0.01, *** p < 0.001, paired t test). c Upper panel, scatter plot of RNAseq results showing gene expression changes 3 h post-LPS stimulation in MTX-M-MØ (LPS + MTX-M-MØ/LPS + M-MØ). The number of annotated genes whose expression is upregulated or downregulated 3 h post-LPS stimulation in M-MØ after 7 days of MTX treatment (adjusted p <0.05) is shown. Lower panel, relative level of expression of the indicated genes as determined by RNAseq on LPS + M-MØ and LPS + MTX-M-MØ, adjusted p value is indicated. d GSEA on the ranked comparison of the transcriptome of LPS + MTX-M-MØ versus LPS + M-MØ, using the genes significantly modulated by LPS in GM-MØ (GM-MØ-specific LPS-induced) and M-MØ (M-MØ-specific LPS-induced) as data set. The genes within the leading edge of each GSEA are indicated in online supplementary Table . e, f Immunoblot analysis of pERK, <t>pJNK,</t> <t>and</t> <t>pp38</t> ( e ), pIRF3 and pSTAT3 ( f ) by monocytes differentiated with M-CSF in the absence or presence of MTX for 7 days and challenged with LPS for the indicated time points. Vinculin or GAPDH protein levels were determined as protein loading control.
Polyclonal Primary Antibodies Against Pstat3 (Tyr705), P P38, Perks, Pjnks, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibody+against+perk/anti+stat3/pmc03389003-327-23-26
Average 90 stars, based on 1 article reviews
polyclonal primary antibodies against pstat3 (tyr705), p-p38, perks, pjnks - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rabbit polyclonal antibodies against perk wlp1512  (Wanleibio)
90
Wanleibio rabbit polyclonal antibodies against perk wlp1512
LPS activated MTX-treated M-MØ exhibit a functional proinflammatory profile. a Experimental design. Monocytes were untreated or exposed to 5 µM MTX at the beginning of the 7-day macrophage differentiation process with M-CSF and challenged with LPS (10 ng/mL) on day 7. Cells were assayed 3 h post-LPS stimulation on M-MØ and MTX-M-MØ. b Production of IL-10, IFNβ, and IL-6 by M-MØ and MTX-M-MØ challenged with LPS for 3 h as determined by ELISA. Mean ± SEM of 10–12 independent donors, each symbol represents a single donor (** p < 0.01, *** p < 0.001, paired t test). c Upper panel, scatter plot of RNAseq results showing gene expression changes 3 h post-LPS stimulation in MTX-M-MØ (LPS + MTX-M-MØ/LPS + M-MØ). The number of annotated genes whose expression is upregulated or downregulated 3 h post-LPS stimulation in M-MØ after 7 days of MTX treatment (adjusted p <0.05) is shown. Lower panel, relative level of expression of the indicated genes as determined by RNAseq on LPS + M-MØ and LPS + MTX-M-MØ, adjusted p value is indicated. d GSEA on the ranked comparison of the transcriptome of LPS + MTX-M-MØ versus LPS + M-MØ, using the genes significantly modulated by LPS in GM-MØ (GM-MØ-specific LPS-induced) and M-MØ (M-MØ-specific LPS-induced) as data set. The genes within the leading edge of each GSEA are indicated in online supplementary Table . e, f Immunoblot analysis of pERK, <t>pJNK,</t> <t>and</t> <t>pp38</t> ( e ), pIRF3 and pSTAT3 ( f ) by monocytes differentiated with M-CSF in the absence or presence of MTX for 7 days and challenged with LPS for the indicated time points. Vinculin or GAPDH protein levels were determined as protein loading control.
Rabbit Polyclonal Antibodies Against Perk Wlp1512, supplied by Wanleibio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibody+against+perk/anti+erk/pm36430869-249-18-25
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibodies against perk wlp1512 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rabbit polyclonal antibody against pfgfr,pplcγ, and perk  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc rabbit polyclonal antibody against pfgfr,pplcγ, and perk
LPS activated MTX-treated M-MØ exhibit a functional proinflammatory profile. a Experimental design. Monocytes were untreated or exposed to 5 µM MTX at the beginning of the 7-day macrophage differentiation process with M-CSF and challenged with LPS (10 ng/mL) on day 7. Cells were assayed 3 h post-LPS stimulation on M-MØ and MTX-M-MØ. b Production of IL-10, IFNβ, and IL-6 by M-MØ and MTX-M-MØ challenged with LPS for 3 h as determined by ELISA. Mean ± SEM of 10–12 independent donors, each symbol represents a single donor (** p < 0.01, *** p < 0.001, paired t test). c Upper panel, scatter plot of RNAseq results showing gene expression changes 3 h post-LPS stimulation in MTX-M-MØ (LPS + MTX-M-MØ/LPS + M-MØ). The number of annotated genes whose expression is upregulated or downregulated 3 h post-LPS stimulation in M-MØ after 7 days of MTX treatment (adjusted p <0.05) is shown. Lower panel, relative level of expression of the indicated genes as determined by RNAseq on LPS + M-MØ and LPS + MTX-M-MØ, adjusted p value is indicated. d GSEA on the ranked comparison of the transcriptome of LPS + MTX-M-MØ versus LPS + M-MØ, using the genes significantly modulated by LPS in GM-MØ (GM-MØ-specific LPS-induced) and M-MØ (M-MØ-specific LPS-induced) as data set. The genes within the leading edge of each GSEA are indicated in online supplementary Table . e, f Immunoblot analysis of pERK, <t>pJNK,</t> <t>and</t> <t>pp38</t> ( e ), pIRF3 and pSTAT3 ( f ) by monocytes differentiated with M-CSF in the absence or presence of MTX for 7 days and challenged with LPS for the indicated time points. Vinculin or GAPDH protein levels were determined as protein loading control.
Rabbit Polyclonal Antibody Against Pfgfr,Pplcγ, And Perk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibody+against+perk/rabbit+polyclonal+antibody+against+pfgfr+pplc%CE%B3++and+perk/pm35436119-415-7-11
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibody against pfgfr,pplcγ, and perk - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


LPS activated MTX-treated M-MØ exhibit a functional proinflammatory profile. a Experimental design. Monocytes were untreated or exposed to 5 µM MTX at the beginning of the 7-day macrophage differentiation process with M-CSF and challenged with LPS (10 ng/mL) on day 7. Cells were assayed 3 h post-LPS stimulation on M-MØ and MTX-M-MØ. b Production of IL-10, IFNβ, and IL-6 by M-MØ and MTX-M-MØ challenged with LPS for 3 h as determined by ELISA. Mean ± SEM of 10–12 independent donors, each symbol represents a single donor (** p < 0.01, *** p < 0.001, paired t test). c Upper panel, scatter plot of RNAseq results showing gene expression changes 3 h post-LPS stimulation in MTX-M-MØ (LPS + MTX-M-MØ/LPS + M-MØ). The number of annotated genes whose expression is upregulated or downregulated 3 h post-LPS stimulation in M-MØ after 7 days of MTX treatment (adjusted p <0.05) is shown. Lower panel, relative level of expression of the indicated genes as determined by RNAseq on LPS + M-MØ and LPS + MTX-M-MØ, adjusted p value is indicated. d GSEA on the ranked comparison of the transcriptome of LPS + MTX-M-MØ versus LPS + M-MØ, using the genes significantly modulated by LPS in GM-MØ (GM-MØ-specific LPS-induced) and M-MØ (M-MØ-specific LPS-induced) as data set. The genes within the leading edge of each GSEA are indicated in online supplementary Table . e, f Immunoblot analysis of pERK, pJNK, and pp38 ( e ), pIRF3 and pSTAT3 ( f ) by monocytes differentiated with M-CSF in the absence or presence of MTX for 7 days and challenged with LPS for the indicated time points. Vinculin or GAPDH protein levels were determined as protein loading control.

Journal: Journal of Innate Immunity

Article Title: GSK3β Inhibition Prevents Macrophage Reprogramming by High-Dose Methotrexate

doi: 10.1159/000526622

Figure Lengend Snippet: LPS activated MTX-treated M-MØ exhibit a functional proinflammatory profile. a Experimental design. Monocytes were untreated or exposed to 5 µM MTX at the beginning of the 7-day macrophage differentiation process with M-CSF and challenged with LPS (10 ng/mL) on day 7. Cells were assayed 3 h post-LPS stimulation on M-MØ and MTX-M-MØ. b Production of IL-10, IFNβ, and IL-6 by M-MØ and MTX-M-MØ challenged with LPS for 3 h as determined by ELISA. Mean ± SEM of 10–12 independent donors, each symbol represents a single donor (** p < 0.01, *** p < 0.001, paired t test). c Upper panel, scatter plot of RNAseq results showing gene expression changes 3 h post-LPS stimulation in MTX-M-MØ (LPS + MTX-M-MØ/LPS + M-MØ). The number of annotated genes whose expression is upregulated or downregulated 3 h post-LPS stimulation in M-MØ after 7 days of MTX treatment (adjusted p <0.05) is shown. Lower panel, relative level of expression of the indicated genes as determined by RNAseq on LPS + M-MØ and LPS + MTX-M-MØ, adjusted p value is indicated. d GSEA on the ranked comparison of the transcriptome of LPS + MTX-M-MØ versus LPS + M-MØ, using the genes significantly modulated by LPS in GM-MØ (GM-MØ-specific LPS-induced) and M-MØ (M-MØ-specific LPS-induced) as data set. The genes within the leading edge of each GSEA are indicated in online supplementary Table . e, f Immunoblot analysis of pERK, pJNK, and pp38 ( e ), pIRF3 and pSTAT3 ( f ) by monocytes differentiated with M-CSF in the absence or presence of MTX for 7 days and challenged with LPS for the indicated time points. Vinculin or GAPDH protein levels were determined as protein loading control.

Article Snippet: Protein detection was carried out using rabbit polyclonal antibodies against pp38, pJNK, and pERK (clones D3F9, 81E11 and D13.14.4E; Cell Signaling, 1/1,000), pIRF3 (clone 4D4G; Cell Signaling, 1/1,000), pSTING (clone D7C3S; Cell Signaling, 1/1,000), STING (clone D2P2F; Cell Signaling, 1/1,000), pCSF1R (clone 49C10; Cell Signaling, 1/1,000), CD209 (dsg-1, 1/1,000) [ ], MAF (sc-7866; Santa Cruz Biotech, 1/1,000), MAFB (clone O91E9; BioLegend, 1/1,000), and pSTAT3 (clone D3A7; Cell Signaling, 1/2,000), goat polyclonal against CSF1R (AF329; R&D Systems, 1/2,000), and mouse monoclonal antibody against human FOLR2 (FRβ, kindly provided by Dr. Takami Matsuyama [ ], 1/800).

Techniques: Functional Assay, Enzyme-linked Immunosorbent Assay, Gene Expression, Expressing, Comparison, Western Blot, Control